Effect of Endocrine Disruptors on the Oocyte Maturation and Ovulation in Amphibians, Rana dybowskii
2007
Choi, M.J. (Chonnam National University, Gwangju, Republic of Korea) | Kim, S.C. (Chonnam National University, Gwangju, Republic of Korea) | Kim, A.N. (Chonnam National University, Gwangju, Republic of Korea) | Kwon, H.B. (Chonnam National University, Gwangju, Republic of Korea) | Ahn, R.S. (Pochon CHA Medical University, Seoul, Republic of Korea), E-mail: [email protected]
Recently, we have shown that some endocrine disruptors, heavy metals, organotins and azoles suppressed steroidogenic enzymes such as P450 side-chain cleavage enzyme (P450scc) and aromatase in bullfrog ovarian follicles. In the present study, by using an amphibian ovarian follicle culture system, we examined the effects of these endocrine disruptors on maturation and ovulation of oocytes from Rana dybowskii in vitro. Ovarian fragments or isolated follicles were cultured for 24 h in a medium containing frog pituitary homogenate (FPH) or progesterone (P₄) with or without endocrine disruptors, and oocyte maturation (germinal vesicle breakdown, GVBD) and ovulation were examined.
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