Effects of sucrose and pH on shoot multiplication of Indian olive (Elaeocarpus robustus Roxb.)
2009
Rahman, Md.M.(Utsunomiya Univ. (Japan). Faculty of Agriculture) | Amin, M.N. | Ishiguri, F. | Yokota, S. | Sultana, R.S. | Takashima, Y. | Iizuka, K. | Yoshizawa, N.
The internode-derived calli of Elaeocarpus robustus were cultured on the shoot regenerating medium, modified MS medium supplemented with 4.4 microM BA and 0.5 microM NAA, with different concentrations of sucrose and pH levels for adventitious shoot regeneration. The results showed that the sucrose concentration at 3% was most suitable for efficient shoot multiplication and pH 5.8 was mostly effective. In the medium supplemented with 3 % sucrose at pH 5.8, the best results were obtained in the frequency of shoot regeneration (90%), number of shoots per culture (6.2 shoots), and average length of shoots per culture (5.3 cm). The higher and lower sucrose concentrations and pH levels were less effective for shoot development in this species.
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