Mass Screening of Lovastatin High-yielding Mutants through Statistical Optimization of Sporulation Medium and Application of Miniaturized Fungal Cell Cultures
2007
Ahn, H.J. (Kangwon National University, Chuncheon, Republic of Korea) | Jeong, Y.S. (Chonbuk National University, Jeonju, Republic of Korea) | Kim, P.H. (Kangwon National University, Chuncheon, Republic of Korea) | Chun, G.T. (Kangwon National University, Chuncheon, Republic of Korea), E-mail: [email protected]
For large and rapid screening of high-yielding mutants of lovastatin produced by filamentous fungal cells of Aspergillus terreus, one of the most important stage is to test as large amounts of mutated strains as possible. For this purpose, we intended to develop a miniaturized cultivation method using 7 ㎖ culture tube instead of traditional 250 ㎖ flask (working volume 50 ㎖). For obtaining large amounts of conidiospores to be used as inoculums for miniaturized cultures, 4 components i.e., glucose, sucrose, yeast extract and KH₂PO₄ were intensively investigated, which had been observed to show positive effect on enhancement of spore production through Plackett-Burman design experiment.
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