Analysis of Semen Parameters, Sperm Activity, and Fertility of Somatic Cell Cloned Hanwoo Bulls
2007
Bae, S.H. (National Institute of Animal Science, RDA, Suwon, Republic of Korea) | Hwang, S.S. (National Institute of Animal Science, RDA, Suwon, Republic of Korea) | Yang, B.C. (National Institute of Animal Science, RDA, Suwon, Republic of Korea) | Go, Y.K. (National Institute of Animal Science, RDA, Suwon, Republic of Korea) | Kim, D.H. (National Institute of Animal Science, RDA, Suwon, Republic of Korea) | Im, G.S. (National Institute of Animal Science, RDA, Suwon, Republic of Korea) | Choi, H.S. (Shinheung College, Uijeongbu, Republic of Korea) | Jin, D.I. (Chungnam National University, Daejeon, Republic of Korea) | Yang, B.S. (National Institute of Animal Science, RDA, Suwon, Republic of Korea) | Seong, H.H. (National Institute of Animal Science, RDA, Suwon, Republic of Korea), E-mail: [email protected]
This study was performed to investigate the reproductive characteristics of the cloned Hanwoo bulls produced by SCNT. The semen ejaculated from the cloned bulls (C-38 and C-39) and normal Hanwoo bull was properly measured the volume, the number of sperm, and the viability of frozen-thawed sperm. The sperm activity was analyzed using computer assisted sperm analysis (CASA). To analyze fertilizing ability of the cloned bulls, in vitro fertilization and artificial insemination were performed using the frozen-thawed semen. There were no differences in semen volume, sperm concentration, and the viability of frozen-thawed sperm between cloned bulls and normal bull. The difference was statistically significant in total motility, curvilinear velocity (VCL), straight-line velocity (VSL), and average-path velocity (VAP) of both cloned bulls compared to those of normal Hanwoo bull, respectively (p less than 0.05). The cleavage and blastocyst development rate were not different between the groups.
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