Antioxidative and neuroprotective effects of enzymatic extracts from leaves of Perilla frutescens var. japonica
2008
Kim, E.K. (Konkuk University, Chungju, Republic of Korea) | Lee, S.J. (Konkuk University, Chungju, Republic of Korea) | Lim, B.O. (Konkuk University, Chungju, Republic of Korea) | Jeon, Y.J. (Cheju National University, Jeju, Republic of Korea) | Song, M.D. (Konkuk University, Chungju, Republic of Korea) | Park, T.K. (Konkuk University, Chungju, Republic of Korea) | Lee, K.H. (Konkuk University, Chungju, Republic of Korea) | Kim, B.K. (Konkuk University, Chungju, Republic of Korea) | Lee, S.R. (Konkuk University, Seoul, Republic of Korea) | Moon, S.H. (Konkuk University, Chungju, Republic of Korea) | Jeon, B.T. (Konkuk University, Chungju, Republic of Korea) | Park, P.J. (Konkuk University, Chungju, Republic of Korea), E-mail: [email protected]
The antioxidative activity of various enzymatic extracts from leaves of Perilla frutescens var. japonica was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. For this study, the leaves were enzymatically hydrolyzed by 8 carbohydrases (Dextrozyme, AMG, Promozyme, Maltogenase, Termamyl, Viscozyme, Celluclast, and BAN) and 9 proteases [Flavourzyme, Neutrase, Protamex, Alcalase, PP-trypsin (trypsin from porcine pancreas), papain, pepsin, α-chymotrypsin, and BP-trypsin (trypsin from bovine pancreas)]. The DPPH radical scavenging activities of Promozyme and Alcalase extracts were the highest, and the IC∧50 values were 77.25 and 109.66 ㎍/mL, respectively. All enzymatic extracts of the leaves scavenged hydroxyl radical, and the IC∧50 values of Celluclast and pepsin extracts which were the highest activity were 243.34 and 241.86 ㎍/mL, respectively. The BAN and α-chymotrypsin extracts showed the highest scavenging activities, and the IC∧50 values were 21.13 and 33.23 ㎍/mL, respectively. The pepsin extracts from the leaves showed protective effect on H₂O₂-induced DNA damage. In addition, the pepsin extracts decreased cell death in PC-12 cells against H₂O₂-induced oxidative damage. The findings of the present study suggest that enzymatic extracts of the leaves possess antioxidative activity.
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