Immunomodulating activities of water-soluble exopolysaccharides obtained from submerged culture of Lentinus lepideus
2008
Jung, Y.S. (Daegu University, Gyeongsan, Republic of Korea) | Yang, B.K. (Daegu University, Gyeongsan, Republic of Korea) | Jeong, Y.T. (Daegu University, Gyeongsan, Republic of Korea) | Islam, Rezuanul (Daegu University, Gyeongsan, Republic of Korea) | Kim, S.M. (Daegu University, Gyeongsan, Republic of Korea) | Song, C.H. (Daegu University, Gyeongsan, Republic of Korea), E-mail: [email protected]
Immunomodulating activities of water-soluble exopolysaccharides (LL-EX) obtained from submerged mycelial culture of Lentinus lepideus were studied and their effectiveness was compared with lipopolysaccharide (LPS). The influence of the LL-EX on macrophage cellular lysosomal enzyme activity was to stimulate up to 267%, 392%, and 464% at the level of 10, 50, and 100 ㎍/ml, respectively. When the LL-EX was further fractionated into LL-Fr.I and Fr.II by Sepharose CL-6B gel chromatography, the cellular lysosomal enzyme activity of LL-Fr.II (2.1-fold) was higher than Fr.I (1.2-fold). Moreover, both LL-Fr.I and Fr.II stimulated the cytokines IL-1β, TNF-α, and IL-6 in macrophages. In mixed lymphocyte reaction, LL-Fr.I and Fr.II enhanced the splenocyte proliferation up to 1.2-fold and 1.4-fold (50 ㎍/ml), respectively, stimulating only T lymphocytes. The fractions of LL-EX not show any direct toxicity against human gastric adenocarcinoma cell (AGS). The molecular masses of LL-Fr.I and Fr.II were estimated to be about 1,986 kDa and 21 kDa, respectively. The total sugar and protein contents of the two fractions were 84.97% and 69.88%, and 15.03% and 30.12%, respectively. The sugar and amino acid compositions of the LL-Fr.I and Fr.II were also analyzed in detail.
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