Reduction of free gossypol levels in cottonseed meal by microbial treatment
2008
Khalaf, M.A. (National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo (Egypt). Radiation Microbiology Dept.) | Meleigy, S.A. (National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo (Egypt). Radiation Microbiology Dept.)
Experiments were carried out to study the effect of some local selected fungi on the reduction of free gossypol (FG) levels during solid-state fermentation (SSF) of cottonseed meal (CSM) and evaluate the most effective strain for biodegradation of FG (BFG) through optimization the parameters of SSF process and also to determine the crude protein (CP) and amino acid (AA) content of CSM substrate fermented under optimal conditions. Results indicated that microbial fermentation could greatly decreased FG levels in CSM, but it differed among species of microorganisms with Candida tropicalis the most effective. The optimum fermentation conditions for BFG by C. tropicalis were incubation period of 48 h, incubation temperature at 30 degree C, inoculum level at 1 x 10(7) cells g-1 of solid substrate, moisture content of solid substrate 55% and pH in nature (5.2). The CP and AA content of the fermented substrate under optimizing conditions were improved markedly.
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