PCR [polymerase chain reaction]-based diagnostic method for livestic trypanosomosis in Quezon [Philippines]
2008
Baticados, W.N., Philippines Univ. Los Banos, College, Laguna (Philippines). Dept. of Veterinary Paraclinical Sciences
A total of 337 horse blood samples from 11 selected municipalities in Quezon province [Philippines] were processed for blood parasite examination (BPE) and polymerase chain reaction (PCR) assay. Using PCR technology, Trypanosoma evansi (T. evansi) infection was detected in 318 (94%) of the total 337 horse blood samples. One hundred seventy-six (55%) of T. evansi positive samples showed two unknown extra bonds slightly higher than the Trypanosoma congolense positive control after multiplex PCR and gel electrophoresis analysis. Among the examines municipalities, Sampaloc (81%) had the most number of T. evansi positive samples with extra PCR bands whereas Alabat (20%) had least. Using PCR, the highest (100%) municipal prevalence was observed for Perez and San Antonio while the least in Alabat (83%). Majority of the infected animals were male horses and 72% (229/318) of the infected animals were 2 years of age. All samples from the different municipalities were found negative for trypanosome after blood parasite examination. On the contrary, results of PCR yielded 80-100% prevalence signifying that infection of the different municipalities were relatively high. This data supports the idea that PCR is a more sensitive and reliable diagnostic method than BPE for the detection of livestock trypanosomes. In addition, the results of PCR had definitively diagnosed the presence of trypanosomosis in the province of Quezon.
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