In vitro callogenesis and organogenesis in different explants of Stevia (Stevia rebaudiana)
2009
Naz, S. (Lahore Coll. for Women Univ., Lahore (Pakistan). Botany Dept.) | Hashmi, A. (Lahore Coll. for Women Univ., Lahore (Pakistan). Botany Dept.) | Ali, A. (Lahore Coll. for Women Univ., Lahore (Pakistan). Botany Dept.)
Different Explants of Stevia rebaudiana i.e. leaf, node and shoot apices were used to callus initiation and regeneration of shoots when cultured on Murashige and Skoog (MS) medium supplemented with different concentration of auxins and cytokinins. Different concentrations of 2, 4-D were used for callus initiation in leaf and nodal explants. In the case of leaf explants the best callus was observed at the concentration of 2,4-D 2.5 mg/l and in nodal explants at the concentration of 1.5 mg/l. Leaf calluses were sub cultured in different concentrations of 2,4-D as well as BAP + IAA for further proliferation of callus and for organ formation. The combination of BAP + IAA (2+1 mg/l) was considered the best medium for organ formation. For shoot apices different concentrations of BAP alone and in combination with IAA were used. BAP 1 mg/l and BAP + IAA (2+1 mg/l) gave the best response. Regenerated shoots were transferred to the rooting medium containing different auxins. Medium containing 0.5 mg/l IBA favored root formation.
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