Comparison of some Indian populations of cereal cyst nematode, Heterodera avenae (Wollenweber, 1924) using RAPD
2008
Abdollahi, M. (Yasouj Univ. (Iran). Dept. of Plant Protection)
Cereal cyst nematode, Heterodera avenae parasitizes the roots of wheat, and is one of the most important and endemic problems in wheat growing belts of India. Knowledge of genetic variabil-ity present among different geographical populations is important for the selection of suitable control strategies. Molecular diversity among seven Indian populations of H. avenae collected from wheat growing areas, Aligarh, Ludhiana, Udaipur, Delhi, Indore, Samastipur and Hisar, has been analyzed by random amplified polymorphic DNA (RAPD). Twenty two random Operon primers of OPA, OPB, OPC and OPD series were used for exploring the polymorphism and gave 315 scorable bands. The number of amplified fragments per primer varied from 10 (with OPD 10) to 94 (with OPD 11). The dendrogram obtained, based on UPGMA clustering of a similarity matrix, indicates that the population of Udaipur forms a separate cluster. Primer OPA 10 amplified a fragment of 3.1 kb only in Udaipur and Delhi populations. Similarly, OPA 3 amplified a 2.75 kb fragment only in Ludhiana population.
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