In-vitro response of potato varieties to various agar concentrations
2007
Khan, S. (NWFP Agricultural Univ., Peshawar (Pakistan). Dept. of Plant Breeding and Genetics) | Raziuddin (NWFP Agricultural Univ., Peshawar (Pakistan). Dept. of Plant Breeding and Genetics) | Jan, H. (Potato Research Center, Abbottabad (Pakistan))
A study was conducted to investigate the in-vitro response of two potato cultivars viz. Desiree and Cardinal to water stress induced by various concentrations of agar (4 to 20 g L-1 with increments of 2 g L-1) supplemented to MS medium. Nine agar concentrations were tested in RCBD with three replications. The results showed that mean square values differed significantly (P less than or equal to 0.05) for genotypes, agar treatments and interaction of these two for root initiation, number of days to transplantation, number of nodes per plantlets, number of leaves per plantlets and number of roots per plantlets. Mean squares were significant only for agar treatments for shoot initiation whereas values were non-significant for plantlets height on transplantation. Variety Desiree surpassed variety Cardinal for shoot initiation, number of leaves per plantlets and number of root per plantlets. Variety Cardinal exceeded variety Desiree for root initiation, number of days to transplantation, number of nodes per plantlets and plantlets height on transplantation. The data indicated that most of the parameters responded favorably to the lower concentrations, however higher agar concentration had detrimental effect and the characters under study were negatively affected. The increased concentrations of agar made the media stiff and created drought like conditions, making the availability of water and nutrients difficult.
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