Paternity testing in dairy cattle based on microsatellite markers | การตรวจสอบความสัมพันธ์ระหว่างพ่อกับลูกโคนมด้วยเครื่องหมายพันธุกรรมชนิดไมโครแซทเทลไลท์
2008
Supitchaya Chethasing(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Science. Department of Animal Husbandry) E-mail:[email protected] | Duangsmorn Suwattana(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Science. Department of Animal Husbandry) | Jutharat Jirasupachok(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Science. Department of Animal Husbandry) | Weerapong Koykul(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Science. Department of Anatomy)
Objectives of this study were to study the polymorphism and to determine the efficiency of microsatellite markers for paternity testing in dairy cattle. Genomic DNA was extracted from frozen semen samples collected from ten sires and from blood samples of there sires's progeny (n=71). Samples in the progeny of 10 sires and frozen semen samples in sires. Each PCR was performed using microsatellite primer for 10 loci (BM1824, ETH10, BM2113, TGLA122, TGLA227, TGLA126, SPS115, ETH152, ETH3 and INRA023). The PCR products were analyzed using polyacrylamide gel electrophoresis (PAGE). The allele frequencies, heterozygosity, polymorphism information content (PIC), exclusion probability (PE) and combine exclusion probability (CPE) were calculated. The results showed that 3-7 type of polymorphism and the range of allele frequencies was 0.007-0.4219. Heterozygosity was range 0.6372-0.7906, the PIC was range 0.5634-0.7598. The range of Probability of Exclusion was 0.3540-0.5914 and accurate for paternity testing (CPE) was highest to 0.9991 (99.91 percent).
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