Extraction and characterization of protein from skim rubber | การสกัดและการวิเคราะห์โปรตีนจากยางสกิม
2009
Nanticha Kalapat(Mahidol University, Bangkok (Thailand). Faculty of Science. Materials Science and Engineering Program) | Ladawan Watthanachote(Mahidol University, Bangkok (Thailand). Faculty of Science. Materials Science and Engineering Program) | Thirawan Nipithakul(Thammasat University. Lampang Centre, Lampang (Thailand). College of Interdisciplinary)
To optimize the extraction of proteins from skim natural rubber (NR), several variables were examined: solution type, number of extractions and sample size. Two solutions, phosphate buffered saline and sodium dodecyl sulfate, have been used for protein extraction. The protein content was determined by Bradford assay. SDS-Polyacrylamide Gel Electrophoresis was employed to check the molecular weight of the protein extract. Analyses of protein by FTIR, UV-Vis spectrometer and CHN analyzer were also carried out for the extract from skim NR in comparison to bovine serum albumin, a model protein. Results revealed that all factors had significant effects on the levels of protein extract. High extractable protein content from the skim NR has been obtained by using small specimen in fresh SDS solution and repeating extraction step to three times (12 hours each). SDS-PAGE demonstrated that the extractable protein had molecular weight of 66 and 116 kDa, corresponding to serum albumin and beta-galactocidase, respectively. Protein impurities could be detected by CHN method, infrared technique and UV absorbance analysis.
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