Simultaneous saccharification and fermentation of cassava pulp to ethanol by co-culture of Rhizopus oryzae and Saccharomyces cerevisiae in solid state culture | การย่อยกากมันสำปะหลังให้เป็นน้ำตาลและการหมักเอทานอลในขั้นตอนเดียวโดยเชื้อผสมระหว่าง Rhizopus oryzae และ Saccharomyces cerevisiae ในการเพาะเลี้ยงแบบ solid state
2009
Thanapoom Maneeboon(Kasetsart University, Bangkok (Thailand). Faculty of Science. Department of Microbiology) E-mail:[email protected] | Ancharida Akaracharanya(Chulalongkorn University, Bangkok (Thailand). Faculty of Science. Department of Microbiology) | Teerapatr Srinorakutara(Thailand Institute of Scientific and Technological Research, Pathum Thani (Thailand)) | Vichien Kitpreechavanich(Kasetsart University, Bangkok (Thailand). Faculty of Science. Department of Microbiology)
Feasibility study on ethanol production from cassava pulp by simultaneous saccharification and fermentation (SSF) with co-culture of Rhizopus oryzae and Sacharomyces cerervisiae in solid state culture was investigation. The ethanol production performed on solid substrate at initial moisture content of 80 percent consisted of 16 g cassava pulp and 4 g corncob by each of four strains of R. oryzae. The result indicated that each culture was able to produce ethanol, particularly R. oryzae TISTR 3165 and TISTR 3523 gave higher ethanol production. Addition of MgSO4.7H2O and KH2PO4 increased the ethanol production. In contrast, no ethanol production was found with addition of nitrogen source. An increase of cassava pulp increased ethanol production. Co-culture of R. oryzae and S. cerervisiae increased ethanol concentration and production rate. Inoculation of S. cerevisiae after 3 days culture of R. oryzae in SSF enhanced the yield and productivity of ethanol 2 folds as compared with the culture inoculated with single culture of R. oryzae.
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