Research in genetic diversity of fusarium species and resistance to fusarium oxysporum cubense in banana varieties
2005
Doan Thi Thanh | Duong Thi Minh Nguyet
Banana has been a traditional and very commonly grown fruit crop in Vietnam, especially in household orchards. There are two major groups, of which the Chuoi Tay group is susceptible to Fusarium oxysporum cubense (FOC), but Chuoi Tieu group is resistant to FOC. A study was carried out on the genetic diversity of Fusarium species associated with banana, rice, potato, tomato, onion, alfalfa, bean, soybean. etc. by AFLP. AFLP analysis was useful in the identification of genetic diversity for 46 different Fusarium species including two groups of clusters: the major cluster was clustered in three first major clusters with 33 percent genetic similarity (GS) or 67 percent polymorphism. This cluster divided into three sub clusters, the first included all F.oxysporum with GS = 34 percent and one cluster branched of F. chlamydosporum with GS = 33percent. The second sub cluster consisted six isolates of F. solani with GS = 30 percent. The third sub cluster included all F. avenaceum isolates with GS = 27 percent. The minor cluster contained all F. moniliforme at the genetic similarity of GS = 26 percent. The results of the AFLP analysis showed great genetic diversity among the Fusarium spp. In terms of species origin, geographical origin conditions. RAPD analysis with the Primer OPA-03 showed a major band of 500 by sharing by five susceptible banana varieties (chuoi Tay, chuoi Ngu, chuoi Ngop, chuoi Hot and chuoi Tay Tia) that was absent in resistant varieties. A band of 1000 by derived from the Primer 21 was observed to be present only in the five resistant banana varieties (chuoi Tieu vua, Tieu Lun. Tieu cao, chuoi Dai Loan, and FHIA-03) but absent in the susceptible ones.
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