Apices and fruit locule number in tomato during floral bud differentiation stage

Chinese. 【目的】研究番茄茎尖内源激素的变化动态,探讨番茄花芽分化期相关内源激素的作用。【方法】应用番茄多心室自交系（MLK1）和少心室自交系（FL1）进行杂交,研究亲本及F1、RF1花芽分化初期、花芽分化期、萼片花瓣形成期、心皮形成初期、子房心室完全形成期5个时期茎尖内源激素GA3、IAA、ABA含量的变化及其与心室数目的关系。【结果】番茄亲本及F1、RF1在关键作用时期的GA3含量高低与各材料的子房心室数变化趋势相同,IAA、ABA含量高低与各材料的子房心室数变化趋势相反。相关分析表明,番茄子房心室数与萼片花瓣形成期GA3含量及GA3/IAA、GA3/ABA呈极显著正相关,而IAA、ABA含量呈极显著负相关。【结论】增加番茄茎尖的GA3含量或降低IAA、ABA的含量,都可以增加番茄子房心室数目;反之则减少子房心室数目;萼片花瓣形成期是调控番茄相关内源激素关键时期。

English. [Objective] The changes of the endogenous hormone of stem apices in tomato, and the functions of relevant endogenous hormone in tomato during floral bud differentiation stage were studied. [Method] Tomato crosses were made by using two inbred lines of multi-locule （MLK1） and few-locule （FL1） with significant difference, the relationship between endogenous hormone GA3, IAA and ABA content and ovary locule number of parents and progeny was studied during floral bud differentiation initial stage, floral bud differentiation stage, sepal petal formation stage, carpel formation initial stage and ovary locule complete formation stage. [Result] GA3 content of P1, P2, F1 and RF1 is consistent with locule number during the key stage, IAA and ABA content is inconsistent with ovary locule number during the key stage. The correlation shows that during the sepal petal formation stage, the ovary locule number is positively correlated with GA3 content and GA3/IAA, GA3/ABA and are negatively correlated with IAA, ABA contents. [Conclusion] It is speculated that increasing the GA content or decreasing the IAA and ABA contents of stem apices in tomato seedling might be able to enhance ovary locule number. The sepal petal formation stage is an important stage which regulates endogenous hormone during ovary locule formation.