Analysis of simple sequence repeats in rhizobium genomes
2008
Gao Yamei | Han Yiqiang | Tang Hui
Chinese. 【目的】分析根瘤菌基因组中的简单重复序列（simple sequence repeats，SSRs），为其在根瘤菌遗传多样性研究中的应用提供有益的信息。【方法】利用公共的微生物串联重复序列数据库资源，对已测序的3种根瘤菌基因组中SSRs的结构类型、分布、丰度等进行系统的比较分析。【结果】大豆慢生根瘤菌（Bradyrhizobium japonicum）、百脉根根瘤菌（Mesorhizobium loti）和苜蓿中华根瘤菌（Sinorhizobium meliloti）基因组中的SSRs分别为1 410个、859个和638个，3种根瘤菌基因组中长重复的四、五、六核苷酸基序更为丰富，变异性更高。数目最少的为单碱基重复。【结论】3种根瘤菌的SSR在结构类型和分布规律上均具有一定的相似性。
Show more [+] Less [-]English. [Objective] Simple sequence repeats （SSRS）or microsatellites, as genetic markers, are ubiquitous in genomes of various organisms. The analysis of SSR in rhizobia genome has provided useful information for a variety of applications in population genetics of rhizobia. [Method] This study examined the SSRs in completely sequenced Bradyrhizobium japonicum, Mesorhizobium loti and Sinorhizobium meliloti genomes in the Microorganisms Tandem Repeats Database. The occurrences, relative abundance, relative density, most common and longest SSRs in the three species were analyzed and compared with each other. [Result] The results revealed that the SSRs number in B. japonicum, M. loti and S. meliloti genomes were 1 410, 859 and 638, respectively. In the three genomes, tetra-, penta- and hexa-nucleotide repeats were more abundant, indicating higher mutation rates in these species. The least abundance was mono-nucleotide repeat. [ Conclusion ] The SSRs type and distribution were similar among these species.
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