Effects of compound Chinese medicinal ingredients on peripheral lymphocyte proliferation, mRNA expression of IFN-γand IL-10 and immunologic effect on rabbit hemorrhagic disease （RHD） vaccine

Chinese. 【目的】研究两个中药成分复方（cCHMIs）的免疫增强作用和机理。【方法】选择黄芪多糖、淫羊藿多糖、蜂胶黄酮和人参皂苷4种中药成分组成两个复方,用MTT法和荧光定量RT-PCR法测定它们对兔外周血淋巴细胞增殖和IFN-γ、IL-10mRNA的表达影响;并将两个复方作为佐剂,配合兔出血症疫苗免疫幼兔,以铝胶苗和无佐剂苗为对照,分别于免疫后7、14、21、35、49d用MTT法和血凝抑制法测定外周血淋巴细胞增殖和血清抗体的动态变化,于免疫后63d测定免疫器官指数和攻毒保护率。【结果】两个复方在体外均可以显著促进淋巴细胞增殖,提高淋巴细胞IFN-γ、IL-10mRNA的表达;作为佐剂,两个复方可以显著提高免疫兔的血清抗体效价,促进体内淋巴细胞增殖,显著促进圆小囊、肠淋巴结等免疫器官的发育,有较高的攻毒保护效果。【结论】两个复方中药成分具有较强的免疫增强作用,可被开发成新型的免疫增强剂。

English. [Objective] The objective of this experiment was to investigate the immune enhancement and mechanism of two cCHMIs. [Method] Two compound Chinese herbal medicine ingredients ingredients （cCHMIs） were prepared with four Chinese herbal medicine ingredients （CHMI）, astragalus polysaccharide （APS）, epimedium polysaccharide （EPS）, propolis flavone （PF）, and ginsenosides （GS）. The effects of the two cCHMIs on lymphocyte proliferation and mRNA expression of IFN-γ, IL-10 were determined by MTT method and fluorescent real-time PCR method. As adjuvant, the two cCHMIs were mixed respectively with RHD vaccine virus to vaccinate rabbits with aluminum and non-adjuvant vaccine as controls. On the 7, 14, 21, 35, and 49 day after vaccination, the dynamic changes of peripheral lymphocyte proliferation and antibody titers were tested by MTT method and HI method, on the 63th day after vaccination, the immune organ and challenge protection were observed. [Result] The two cCHMIs could significantly enhance lymphocyte proliferation, enhance mRNA expression of IFN-γ, IL-10. As adjuvant, the two cCHMIs could significantly enhance serum antibody titers, promote lymphocyte proliferation, stimulate the organ index of Sacculus Rotundus and intestinal lymph node, and present higher protective effect for challenge rabbits. [Conclusion] Two cCHMIs have stronger immune enhancement and can be expected to exploit into new-type of immunopotentiators.