Antioxidation analisis of extracts from petals of 6 tree peonies in vitro | 6个品种牡丹花瓣的抗氧化活性分析

Chinese. 分析不同品种牡丹花瓣的抗氧化活性。采用卵黄脂蛋白过氧化法、DPPH法和氮兰四唑（NBT）光还原法、水杨酸羟基化法，以Vc和BHT为阳性对照，在DPPH 、O2－• 和•OH的产生和检测系统以及卵黄组织匀浆中，加入一定量的牡丹花瓣提取液，观察其与活性氧引发反应的竞争效应，研究6个牡丹品种（“藏枝红”、“洛阳红”、“胡红”、“迎日红”、“赵粉”和“凤丹”）花瓣提取液的抗氧化活性。牡丹花瓣提取液能明显抑制卵黄组织匀浆的脂质过氧化作用，降低DPPH的吸光值、抑制O2－• 介导的NBT光化学还原及•OH 作用下的水杨酸羟基化作用，其中牡丹花瓣提取液清除DPPH自由基的活性高于200 μg/mL BHT，但低于80 μg/mL Vc。6个品种的牡丹花瓣提取液，在4种测定系统中均具有一定程度的抗氧化能力，且水提取液的抗氧化活性略高于体积分数50%乙醇提取液，深色花瓣提取液对不同自由基的清除能力较强。牡丹花瓣可能成为一种新的高效天然抗氧化剂。

English. To extract the antioxidant compounds from peony flowers, its effect of cleaning oxygen radical was analyzed. The extracts from 6 peonies petals were isolated by water and 50% ethanol. Its antioxidation activities were detected and evaluated by four systems of DPPH, O2－•, •OH and PUFA with controls for Vc and BHT．Water or 50% ethanol extracts of 6 peonies petals were added to the generating and detecting systems of DPPH radical，hydroxyl radical (•OH), superoxide radical (O2－•) and PUFA peroxidation of yolk lipoprotein for the observation of its competitive abilities to active reactions. The results indicated that the water or 50% ethanol extracts from peonies petals could reduce light absorption of DPPH and •OH initiated hydroxylation of salicylate, and inhibit O2－• mediated light chemical reduction of nitroblue tetrazolium (NBT) effectively, and lipid peroxidation of polyunsaturated fatty from yolk lipoprotein, in which DPPH scavenging activities of 6 peonies water extracts were higher than that of 200 μg/mL BHT, but lower than 80 μg/mL Vc. In 4 kinds of detecting systems of antioxidation, the peroxidation activity of deep colored flowers of peonies was higher than that of light colored flowers of peonies. The peony flower can be used as an antioxidation．