Formulation of biocontrol actinomyces strain 153 and its control effect on Verticillium wilt | 生防放线菌153活菌剂填料的选择及其防病促生作用研究

Chinese. 筛选153活体菌剂的理想填料，以延长153制剂的货架期，提高其大田防病效果。以固体发酵培养153，分别添加硅藻土、高岭土和非耕作层黄土3种填料，通过活菌量测定和温室防病促生效果观察对理想填料进行筛选。在3种填料中，以加入非耕作层黄土的活体粉剂的存储时间最长，35 ℃下可以保存70 d以上，活菌量保持水平最高。菌株153对茄子的生长发育有明显促进作用，对植株株高、倒3叶叶面积、干质量及根生长的促进作用最为明显，植株鲜质量的增幅最高可达98.47%，地上部干质量和根干质量的增幅最高分别可达74.86%和82.64%。以非耕作层黄土为填料的菌剂对茄子黄萎病的防效可达82.44%。通过加入合适的填料，能有效延长菌剂贮存期并提高菌株的生态适应性，为生防菌153从实验室走向生产实践奠定了基础。

English. The study was done to prolong the shelf life of actinomycetes strain 153 and improve the bio-_control effect in the field grown condition. 153 solid-state fermented culture was mixed with diatomite, kaolin as well as non-cultivated loess and screened by the number of living bacteria and their protection effect on eggplant against Verticillium dahliae was studied. Non-cultivated loess was better than diatomite, kaolin. With this additive, the biocontrol agent could be stored more than 10 weeks at 35 ℃. The growth and development of eggplant could be promoted obviously by live-cell formulation of 153. Especially, the amplitude of green plant weight attained 98.47%, amplitude of dry weight of stem and dry weight of radix attained 74.86% and 82.64%. The control rate of eggplant Verticillium wilt with the formulation mixed non-cultivated loess reached 82.44%. By adding suitable additive, the formulation of actinomyces strain 153 can obtain longer shelf life and better environment adaptation.