Study on the extraction of polyphenol from grapevine branch by ultrasonic adjunct extraction method | 葡萄枝条中多酚类物质的超声波辅助提取

Chinese. 探讨利用超声辅助技术从葡萄枝条中提取多酚类物质的最佳工艺条件，并比较8个酿酒葡萄品种枝条中多酚类物质含量的差异。对影响葡萄枝条中多酚类物质提取率的乙醇溶液体积分数、浸提温度、料液比（质量（g）与体积（mL）比）、提取时间和提取次数进行单因素试验，并在此基础上设计4因素3水平的正交试验，以确定超声波提取的最佳工艺条件；利用最佳工艺条件提取8个葡萄品种枝条中的多酚类物质，并对其含量进行测定。超声波辅助提取葡萄枝条中多酚类物质的最佳条件为：以体积分数60%乙醇溶液为提取液，料液比为1∶12，超声提取时间为30 min，于20 ℃下浸提2次。在最佳工艺条件下，多酚类物质的提取率为97.38%。8个酿酒葡萄品种枝条中的多酚类物质含量均大于20 mg/g，其中品丽珠含量最高（32.855 4 mg/g），其次为梅尔诺（31.967 7 mg/g），以8804最低（20.815 6 mg/g）。确定了超声波辅助提取葡萄枝条中多酚类物质的最佳工艺条件，在此工艺条件下提取率最高可达97.38%。

English. The aim of the study was to investigate the best extracting condition of polyphenol from grapevine branch by ultrasonic adjunct extraction method. And the different contents of polyphenol from eight grapevine cultivars' branches were compared via this method. Influences of extracting power of polyphenol were investigated by single factor test. The infected factors were ethyl alcohol density, extracting temperature, ratio of solid to liquid, ultrasonic time and extracting times. While the orthogonal test was devised to determine the best extracting condition based on the single factor experiment, the polyphenol content of annual grapevine branches that belonged to eight different varieties was reviewed by the extracting conditions. The best extracting condition of polyphenol from grapevine branch by ultrasonic adjunct extraction method was as follows: 60% ethanol as solvent, ratio of solid to liquid 1∶12, ultrasonic time 30 min, extracting temperature 20 ℃ and extracting times 2. And the yield of total polphenol was 97.38%. The polyphenol content of one-year-old grapevine branches that belonged to eight different varieties exceeded 20 mg/g. The content of polphenol of the Cabernet Franc (32.855 4 mg/g) was the highest in the eight different varieties, the Merlot (31.967 7 mg/g) lower, the 8804 the lowest (20.815 6 mg/g). The suitable conditions of polyphenol from one-year-old grapevine branches by ultrasonic adjunct extraction method were determined. And the yield of total polphenol was 97.38%.