Effect of N, N-Dimethylformamide on cryopreservation of boar semen | N,N-二甲基甲酰胺（DMF）对猪精液冷冻效果的影响

Chinese. 研究N, N-二甲基甲酰胺（N,N-Dimethylformamide, DMF）与甘油配制的混合保护剂对猪冷冻精子的保护效果。以DMF和甘油配制成混合冷冻保护剂对猪精液进行冷冻，试验共设对照组（30 mL/L甘油）、A组（10 mL/L DMF+20 mL/L甘油）、B组（20 mL/L DMF+10 mL/L甘油）、C组（30 mL/L DMF）和D组（50 mL/L DMF）5个处理组，以活动精子百分率、精子活率、精子直线运动速率、精子顶体完整率以及精子质膜完整率为检测指标，对猪精子冷冻效果进行评价。20 mL/L DMF + 10 mL/L甘油混合冷冻保护剂对猪精子有较好的冷冻保护作用，与其他处理组相比，除精子直线运动速率和质膜完整率与10 mL/L DMF +20 mL/L甘油组无显著差异外，其他各项指标均优于其他组及甘油对照组。30 mL/L DMF对猪精子的毒性强于同浓度甘油，但20 mL/L DMF+10 mL/L甘油混合冷冻保护剂的毒性与甘油对照组无显著差异（P0.05）。20 mL/L DMF+10 mL/L甘油混合冷冻保护剂比单一使用甘油或DMF能起到更好的冷冻保护作用。

English. The effect of mixed cryoprotectants composed of different proportions of glycerol and N, N-Dimethylformamide (DMF) on freezing boar sperm was studied. The mixing cryoprotectant was composed of DMF and glycerol, and experiments were executed among five treatments: the control (30 mL/L glycerol), group A (10 mL/L DMF +20 mL/L glycerol), group B (20 mL/L DMF +10 mL/L glycerol), group C (30 mL/L DMF) and group D (50 mL/L DMF). In the present study, the effects of mixing cryoprotectant on frozen-thawed sperm were evaluated by five sperm parameters including the percentage of total motile sperm, motility, velocity of straight-line motion, plasma membrane integrity and acrosome integrity. 20 mL/L DMF + 10 mL/L glycerol could protect boar sperm against cryodamage during cryopreservation. No difference on velocity of straight-line motion and membrane integrity of frozen-thawed sperm was found between group B and group A (P0.05), and the parameters of frozen-thawed sperm of the group B were higher than those of other groups (P0.05); The toxicity of DMF was stronger than that of glycerol at the same level of concentration (30 mL/L), and no difference existed between group B and the control in the present study (P0.05). 20 mL/L DMF + 10 mL/L glycerol could provide better protective action for frozen boar sperm than that of DMF or glycerol alone.