Ananlysis of the karyotype and G banding of chromosomes in Lushi green eggshell chicken | 卢氏绿壳蛋鸡染色体核型与G带分析

Chinese. 探讨卢氏绿壳蛋鸡染色体核型和G带带型。采用骨髓法制备卢氏绿壳蛋鸡染色体标本片，染色后对其染色体核型和G带进行分析。卢氏绿壳蛋鸡的体细胞染色体数为2n＝78，该鸡种的核型中有10对大染色体和29对微小染色体，且微小染色体基本为端着丝粒染色体。公鸡染色体核型由38对常染色体和1对同配型性染色体ZZ组成，母鸡由38对常染色体和1对异配型性染色体ZW组成。1、2号和Z、W均为中央着丝粒(m)染色体，4号和7号均为亚中央着丝粒(sm)染色体，3、6、8、9、10号均为端着丝粒(t)染色体。前10对大染色体（包括Z、W染色体）的G带共可分为32个区，118条带。卢氏绿壳蛋鸡的染色体数目为：2n＝78，公鸡核型78，ZZ；母鸡核型78，ZW。卢氏绿壳蛋鸡与其他品种鸡的G带存在一定的差异。

English. The research is to study the karyotype and G-banding pattern of the chromosomes in Lushi green eggshell chicken. The chicken's chromosome samples were prepared by bone marrow method, and Lushi green eggshell chicken’s karyotype and G band patterns after dyeing were analyzed. The number of chromosomes Lushi green eggshell chicken is 2n＝78, the chicken breed with 10 pairs of macro-chromosomes and 29 pairs of micro-chromosomes, and the micro-chromosomes are almost telocentrics chromosome. Male karyotype consists of 38 pairs of chromosomes and one pair of homogametic sex chromosomes ZZ, while female karyotype consists of 38 pairs of chromosomes and one pair of heterogametic sex chromosomes ZW. Lushi green eggshell chicken's No. 1, 2 and Z, W chromosomes are the metacentrics (m) chromosome, No. 4 and No. 7 chromosomes are submetacentrics (sm) chromosome, No. 3, No. 6, No. 8, No. 9 and No. 10 are telocentrics chromosome. The first 10 pairs of macro-chromosomes can be divided into total 32 zones and 118 G bands. The number of the chromosomes in Lushi green eggshell chicken is 2n=78, the karyotypes of male and female chicken are 78, ZZ and ZW respectively. The G banding patterns is different from other breeds.