Effect of LYC-b gene antisense expression on lycopene content | LYC-b基因反义表达对番茄果实番茄红素含量的影响
2009
Xu Jiaxin, Northernwest Agriculture and Forestry University, Yangling (China), College of Horticulture | Liang Yan, Northernwest Agriculture and Forestry University, Yangling (China), College of Horticulture
Chinese. 研究番茄红素β-环化酶（LYC-b）基因反义表达对番茄果实中番茄红素含量的影响，为番茄品质育种提供新方法。以番茄品种“TTI1117A”和“灵光3号”为材料，构建了分别由CaMV35S启动子驱动的GUS标记基因和反义LYC-b 5′基因双价植物表达载体，通过农杆菌GV3101介导，将基因整合到番茄基因组中，采用GUS、PCR以及RT-PCR、Northern 杂交进行检测，并测定了转基因植株果实中的番茄红素含量。由GUS、PCR检测结果可知，筛选出1株“TTI1117A”和4株“灵光3号”转基因植株；RT-PCR、Northern杂交检测结果表明，目的基因在RNA水平上已经表达；对番茄果实中番茄红素含量的测定结果表明，转基因植株果实中番茄红素含量均高于未转基因植株。LYC-b基因的反义表达具有提高番茄果实中番茄红素含量的作用。
Show more [+] Less [-]English. The study discussed the effect LYC-b gene antisense expression on lycopene content in order to provide new methods for the fruit quality breeding of tomato. The two gene plant expression vectors including anti-LYC-b 5′gene and GUS gene were constructed by using CaMV35S as a promoter and introduced into tomato cultivars TTI1117A and Lingguang No. 3 by Agrobacterium tumefaciens GV3101 mediated transformation. GUS, and PCR detection showed that anti LYC-b5′ gene had integrated into genome of tomato. RT-PCR, and Northern blot detection indicated that anti LYC-b5′gene was expressed at RNA level. Lycopene content analysis of tomato fruit showed that lycopene content in transgenic fruit was higher than that of untransgenic fruit. LYC-b gene antisense expression can improve lycopene content of tomato fruit.
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