Determination of purine content in soybean by HPLC | HPLC法测定大豆中嘌呤含量的研究
2009
Liu Shaolin, Anhui Agricultural University, Hefei(China), Shool of Tea and Food Science and Technology | Li Meiqing, Anhui Agricultural University, Hefei(China), Shool of Tea and Food Science and Technology | Ding Zhien, Anhui Agricultural University, Hefei(China), Shool of Tea and Food Science and Technology
Chinese. 建立大豆中多组分嘌呤组分的高效液相色谱分析方法。通过高效液相色谱分析技术,确定水解条件以及色谱分离条件。样品确定的色谱分离条件为：Waters symmetryshield RP18（4.6 mm×150 mm,5.0μm）色谱柱,以0.02 mol.L-1磷酸二氢钾缓冲液（pH4.6）作为流动相,流速0.6 mL.min-1,柱温25℃,进样量5μL,检测波长（λ1）为254 nm;当流动相pH为4.6时,各组分嘌呤得到完全分离,腺嘌呤、鸟嘌呤、黄嘌呤和次黄嘌呤的含量分别为0.732 3、0.821 2、0.020 2和0.031 8 mg.g-1;但回收率不高,分别为68.90%、88.37%、57.19%和68.16%;样品的最佳水解时间为0.5 h。[著者文摘]
Show more [+] Less [-]English. A high performance liquid chromatographic method（HPLC） was developed for determination of purines in soybean.The conditions for hydrolyzation of soybean and separation of several purines by HPLC were optimized.The optimized chromatography conditions were as follows：waters symmetryshield RP18 column was used UV detector was employed at wavelength of 254 nm with 0.02 mol・L. 1 KH2PO4 buffer solution（pH4.6） as mobile phase at flow rate of 0.6 mL・min. 1;and the column temperature was at 25℃. The result shows that several purines could be separated effectively when the pH value was 4.6 and the content of adenine, guanine, xanthine and hypoxanthine were 0. 732 3,0. 821 2,0. 020 2 and 0. 031 8 mg・g. 1, respectively. But the recoveries were poor. Their average recoveries were 68.90%, 88.37% ,57.19% and 68.16%, respectively. The optimal hydrolyzation time was 30 min.
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