Effect of Xiong- gui -jiao - ai tang on Th1/Th2 cytokines of abortive mice induced by RU486 | 芎归胶艾汤对RU486致流产小鼠Th1／Th2型细胞因子的影响

Chinese. 为了研究芎归胶艾汤的促孕保胎作用及其对母胎界面Th1／Th2型细胞因子的影响，取怀孕小鼠分成4组，分别为对照组、RU486组、RU486＋孕酮组、RU486＋芎归胶艾汤组。除对照组外，其它3组于孕4d（GD4）7：00皮下注射RU4860．5mL，同时孕酮组注射0．1mL孕酮，中药组灌服芎归胶艾汤0．8mL，各组分别在GD4下午19：00，GD5上午7：00，GD5下午19：00取材。统计胚泡数，酶联免疫吸附法（ELISA）测定子宫匀浆中IFN-γ、IL-2、IL-4和IL―10的含量。结果表明：RU486显著降低胚泡数，Th1型细胞因子IFN-γ和IL-2的水平显嗜升高，Th2型细胞因子IL-4和IL-10的含量显著下降。补充孕酮或芎归胶艾汤显著逆转了RU486引起的流产，同时降低了Th1型细胞因子水平，提高了Th2型细胞因子水平，表明芎归胶艾汤能够作用在母胎界面，调节着床和妊娠期细胞因子平衡，利于胚胎着床和妊娠维持。[著者文摘]

English. To investigate the effect of Xiong- gui- jiao-ai tang （XGJAT） on abortion model and its impact on Th1/Th2 cytokines at the maternal - fetal interface, the pregnant mice were divided into 4 groups： control group, RU486 group, RU486 ＋ progesterone （P） group and RU486 ＋ XG- JAT group. All mice except the control were injected with 0.5 mL RU486 subcutaneously on day 4 of pregnancy to establish abortion model. At the same time, the mice in RU486 ＋ P group were in-jetted with 0.1 mL progesterone and those of RU486 ＋ XGJAT group gavaged with 0.8 mL Xiong -gui-jiao-ai tang. Samples were collected in 19：00 on day 4, 7：00 and 19：00 on day 5. The number of embryos were calculated and the contents of IFN -γ, IL- 2, IL - 4 and IL - 10 in uterine supernatant were measured by Enzyme- Linked Immuno- absorbent Assay （ELISA）. Results showed that, with decreased number of embryos, the contents of IFN -γ and IL- 2 which represent Thl cytokines significantly increased, while Thl cytokines, such as IL- 4 and IL- 10, significantly decreased after treatment with RU486. Supplement with progesterone or Xiong - gui - jiao- ai tang efficiently attenuated the abortive effect of RU486 through the decreasing of Thl cytokines and increasing the Th2 cytokines, indicating that Xiong- gui- jiao- ai tang modulates cytokine network in pregnancy and benefits embryo implantation and survival.