Effect of germinal vesicle translation process on in vitro fertilization of mouse oocytes | 生发泡移植过程对小鼠卵母细胞体外受精的影响
2009
Cui Longbo, Yantai University, Yantai(China),Department of Biology | Zhou Xueying, Yantai University, Yantai(China),Department of Biology | Cui Zhifeng, Yantai University, Yantai(China), Ocean School
Chinese. 为研究生发泡（GV）移植后的重组卵母细胞能充分在体外成熟和发育,本试验比较了GV移植过程及体外成熟对小鼠卵母细胞体外受精的影响。通过透明带切口及GV移植获得的重组GV期卵母细胞的成熟率为87.0%（147/169）,体外受精后发育到原核期胚和2-细胞期胚的比率分别为76.2%（112/147）和42.2%（62/147）,与仅做透明带切口但不进行GV移植的对照组相似（P〉0.05）,表明GV移植过程未对卵母细胞体外受精后的早期胚胎发育能力产生不利影响。但与体内成熟的卵母细胞相比,体外成熟卵母细胞的受精率和卵裂率都明显降低,表明其进一步发育的能力受到影响。[著者文摘]
Show more [+] Less [-]English. To study oocytes reconstructed by germinal velop fully in vitro, effects of GV translation process vesicle （GV） translation can mature and deand in vitro maturation on in vitro fertilization （IVM） of mouse oocytes were compared. The maturation rate of the reconstructed oocytes obtained by zona cutting and GV translation was 87.0% （147/169）, and the rates of pronuclear and 2-cell embryo developed from these oocytes after IVF were 76.2% （112/147）and 42.2% （62/ 147）, respectively, which were comparable （P 〉0.05） with the control oocytes obtained by zona cutting but not GV translation. The results showed the GV translation process had no harmful effect on early embryo developmental capacity of the oocytes after IVM. The rates of fertilization and cleavage of the oocytes matured in vitro, however, were significantly lower than that matured in vivo, showing the further developping capacity of the oocytes matured in vitro was affected.
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