Establishment of highly efficient regeneration system with thin cell layer of lily scale | 百合鳞片薄层细胞培养高效再生体系的建立
2009
Li Xiaoyan, China Agricultural University ,Beijing(China), College of Agriculture and Biotechnology | Chen Li, China Agricultural University ,Beijing(China), College of Agriculture and Biotechnology | Xin Haibo, China Agricultural University ,Beijing(China), College of Agriculture and Biotechnology
Chinese. 为了建立适宜百合遗传转化的受体系统，以铁炮百合品种白天堂无菌苗的叶片和鳞片为外植体，薄层切割后接种于添加不同植物激素的培养基上，观察其植株再生情况，并对其建立的再生体系的遗传转化前景进行了分析比较。结果表明，叶片薄层细胞几乎没有再生能力；2，4-D和Picloram均可诱导横向薄层鳞片细胞直接产生鳞茎芽，最适的培养基是MS＋2，4-D0．002mg／L，在30d内就可直接诱导出鳞茎芽，再生率达到100％，平均出芽数为2．38；MS＋TDZ 0．4mg／L＋NAA 0．5mg／L可以诱导横向薄层鳞片细胞产生愈伤。综合考虑，百合鳞片薄层培养以MS＋2，4-D 0．002mg／L诱导的直接再生体系为最佳的再生体系。[著者文摘]
Show more [+] Less [-]English. In order to establish the genetic transformation system of lily,the thin cell layer of leaves and scales were cultured on MS media supplemented with 2,4-D, TDZ, NAA or Picloram to screen the suitable explants and plant growth regulators. The results showed that the TCL of leaves was hardly regenerate. 2,4-D and Picloram could induce bulblets from tTCL of scale. The best medium was MS＋2, 4-D 0. 002 mg/L with bud regeneration rate of 100% and average buds of 2. 38 in 30 days. MS＋TDZ 0. 4 mg/L＋NAA 0. 5 mg/L could induce callus from tTCL of scale. On all accounts, the highly efficient regeneration system is to directly regenerate the tTCL of scale with MS＋2,4-D 0. 002 mg/L.
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