Process optimization for enzymatic hydrolysis of hemoglobin in sika deer blood | 鹿血血红蛋白酶水解工艺条件的优化

Chinese. 以静脉采集的抗凝梅花鹿血为材料，考察了蛋白酶种类、酶解条件对鹿血中血红蛋白水解度、氮收率和血红素含量的影响，优化了碱性蛋白酶（Alcalase）和风味蛋白酶（Flavorzyme）的分步酶解工艺，以获得具有良好感官品质和富含血红素的鹿血酶解产品。结果表明：蛋白酶种类和酶解方式对鹿血血红蛋白水解效果有明显影响，采用Alcalase和Flavorzyme分步水解的效果最好。Alcalase和Flavorzyme的最适酶解温度分别为55℃和50℃，最适酶解起始pH值分别为8．0和6．5，2种酶的适宜底物浓度为8％（m／m），适宜加酶量为6000U／g。采用分步酶解工艺时，在底物浓度8％、酶解温度55℃、起始pH值8．0的条件下，先用4500U／g的Alcalase酶解1h，再用1500U／g的Flavorzyme酶解3h，可获得最高的水解度、氮收率和血红素含量，其值分别为27．95％、73．75％和3．326mg／mL。[著者文摘]

English. The anticoagulant venous sika deer blood was hydrolyzed by different enzymes and hydrolysis conditions to examine hydrolysis degree, nitrogen recovery and content of heroin in hemoglobin hydrolysate. Step hydrolysis process with Alcalase and Flavorzyme was optimized to get good quality and hemin enriched hydrolysis product. Enzyme type and enzymatic hydrolysis mode had obvious influence on hydrolysis degree and nitrogen recovery. Step hydrolysis with Alcalase and Flavorzyme obtained a significant effect on sika deer blood. The suitable hydrolysis temperatures of Alcalase and Flavorzyme were 55 ℃ and 50 ℃ ,and initial pH values were 8.0 and 6.5,respectively. For Alcalase and Flavorzyme,the suitable substrate concentration and proteases dosage were 8% and 6 000 U/g, respectively. When 8% substrate（pH 8.0） was hydrolyzed by 4 500 U/g of Alcalase for 1h and followed by 1500 U/g of Flavorzyme for 3 h, the hydrolysis degree, nitrogen recovery and hemin content of hydrolysate were 27. 95% ,73. 75% and 3. 326 mg/mL,respectively.