Sequence analysis of polymorphic fragments in the third intron of porcine H-FABP gene | 猪H-FABP基因内含子3多态片段的序列分析
2009
Yang Wenping, North China Coal Medical University, Tangshan (China), Department of Biological Science | Li Chao, North China Coal Medical University, Tangshan (China), Department of Biological Science | Zhang Hongmei, North China Coal Medical University, Tangshan (China), Department of Biological Science
[Objective] The aim of this study was to provide a theoretical basis for exploring the major genes affecting intramuscular fat (IMF) deposition. [Method] Taking 383 pigs from five breeds including Mashen Pig, Large White Pig, Landrace, Duroc and Shanxi White Pig as the experimental animals, polymorphisms of partial fragments in the third intron of porcine H-FABP gene were detected by PCR-SSCP method, and then the polymorphic fragments were sequenced. [Result] Two alleles, designated as A and B, were found at the locus 346 in the third intron of porcine H-FABP gene, and the mutation was caused by a A→G substitution. [Conclusion] A polymorphic locus was discovered in the third intron of porcine H-FABP gene in this experiment, laying a foundation for the further study on the relationship between H-FABP gene and IMF content.
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