Spore-forming bacteria in soil cultivated with GM white poplars: Isolation and characterization
2010
Bonadei, M.,University of Pavia (Italy). Dept. of Genetics and Microbiology | Calvio, C.,University of Pavia (Italy). Dept. of Genetics and Microbiology | Carbonera, D.,University of Pavia (Italy). Dept. of Genetics and Microbiology | Galizzi, A.,University of Pavia (Italy). Dept. of Genetics and Microbiology | Quattrini, E.,University of Milan, Tavazzano (Italy). C.E.T.A.S. | Balestrazzi, A.,University of Pavia (Italy). Dept. of Genetics and Microbiology
The impact of transgenic white poplars (cv. Villafranca) was assessed on the soil aerobic spore-forming bacteria (SFB). The genetically modified poplars, expressing either the StSy gene for resveratrol production or the bar gene for herbicide tolerance, were cultivated in greenhouse. The occurrence of SFB was monitored in soil samples collected at eight different timepoints over a two-year period. The total culturable bacterial population of the StSy and bar trials underwent significant seasonal fluctuations. Changes occurred also within the culturable SFB population in the StSy and bar trials, respectively. No significant differences in the size of the total and SFB culturable populations were observed when comparing each transgenic line with the nontransformed control line while seasonal shifts of soil bacterial populations were evident in both trials. The culturable SFB fraction included three isolates (SFB-1, SFB-2 and SFB-3) classified by 16S rDNA sequence analysis as members of the Bacillus genus. According to the reported data, cultivation of both herbicide-resistant and resveratrol-producing GM white poplars did not affect the culturable SFB population at the soil level.
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