Isoenzyme polymorphism of almond genotypes selected in the region of northern Serbia
2010
Colic, S.,Institute for Science Application in Agriculture, Belgrade (Serbia) | Milatovic, D.,University of Belgrade (Serbia). Faculty of Agriculture | Nikolic, D.,University of Belgrade (Serbia). Faculty of Agriculture | Zec, G.,University of Belgrade (Serbia). Faculty of Agriculture
Isoenzyme polymorphism was studied in 20 almond genotypes selected from seedling populations of unknown almond cultivars in the region of northern Serbia (Vojvodina). Fourteen enzyme systems were studied using the method of vertical polyacrylamide gel electrophoresis. Ten systems were polymorphic in twelve loci. This polymorphism allowed unique identification of all studied genotypes. The most useful enzyme for analysis of almond genetic variability was menadione reductase. Polymorphism identified for alkaline phosphatase, formate dehydrogenase, glutamate dehydrogenase, malic enzyme, and menadione reductase was reported for the first time in almond. Cluster analysis was used to construct a dendrogram on which five clusters with different number of genotypes could be identified.
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