Somatic embryogenesis from rhizome explants of Cymbopogon winterianus
2010
Dey, T.,University of Kalyani (India). Dept. of Botany | Bhattacharya, S.,University of Kalyani (India). Dept. of Botany | Ghosh, P.D.,University of Kalyani (India). Dept. of Botany
Plantlet regeneration through indirect somatic embryogenesis was attempted from rhizome derived callus of Cymbopogon winterianus (cv. Jorlab2). Optimum callus was induced on Murashige and Skoog (MS) basal medium supplemented with 4 mg/cubic dm 2,4-dichlorophenoxyacetic acid (2,4-D). Initially the callus was friable, shiny white and watery in nature. After subculturing on MS medium containing 2,4-D and kinetin (Kn), callus was transferred onto the MS medium supplemented with 2,4-D, Kn and coconut water to induce somatic embryogenesis. Optimum somatic embryogenesis (78.33 %) was achieved on MS medium containing 3.0 mg/cubic dm 2,4-D and 0.5 mg/cubic dm Kn. High frequency (65 %) plantlet conversion from embryos was achieved in MS medium supplemented with 2 mg/cubic dm N6-benzyladenine, 0.5 mg/cubic dm Kn, 0.2 mg/cubic dm calcium pantothenate and 0.2 mg/cubic dm biotin.
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