Proline accumulation and the expression of delta1-pyrroline-5-carboxylate synthetase in two safflower cultivars
2010
Thippeswamy, M.,Sri Krishnadevaraya Univ., Anantapur (India). Dept. of Botany | Chandraobulreddy, P.,Sri Krishnadevaraya Univ., Anantapur (India). Dept. of Botany | Sinilal, B.,Sri Krishnadevaraya Univ., Anantapur (India). Dept. of Botany | Shiva Kumar, M.,Sri Krishnadevaraya Univ., Anantapur (India). Dept. of Botany | Chinta Sudhakar,Sri Krishnadevaraya Univ., Anantapur (India). Dept. of Botany
Proline (Pro) accumulation under water stress was measured in Carthamus tinctorius drought tolerant cv. A1 and sensitive cv. Nira. Activities of pyrroline-5-carboxylate reductase (P5C reductase) and pyrroline-5-carboxylate synthetase (P5C synthetase), two enzymes involved in the Pro biosynthetic pathway were also estimated. Water stress resulted in a reduction in the leaf dry mass and chlorophyll content along with a gradual accumulation of Pro. RT-PCR results show higher expression of delta1-pyrroline-5-carboxylate synthetase (p5cs) gene in correlation with up-regulated Pro accumulation in cv. A1. P5C reductase was found to be the Pro synthesis rate limiting whereas P5C synthetase did not show any specific response to the drought stress in both cultivars.
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