Impact of gene expression of chosen genes involved in the biosynthetic pathway of vitamin E on the total vitamin E content and composition in grain of spring barley
2010
Kosar, M.,Mendelova Univ., Brno (Czech Republic). Ustav Pestovani, Slechteni Rostlin a Rostlinolekarstvi | Holkova, L.,Mendelova Univ., Brno (Czech Republic). Ustav Pestovani, Slechteni Rostlin a Rostlinolekarstvi | Brezinova Belcredi, N.,Mendelova Univ., Brno (Czech Republic). Ustav Pestovani, Slechteni Rostlin a Rostlinolekarstvi | Ehrenbergerova, J.,Mendelova Univ., Brno (Czech Republic). Ustav Pestovani, Slechteni Rostlin a Rostlinolekarstvi
The highest vitamin E content among cereals was detected in barley. There are two enzymes localized in the strategic location of biosynthetic pathway of vitamin E : 4-hydroxyphenylpyruvate dioxygenase enzyme (HPPD) and homogentisategeranylgeranyl transferase (HGGT). HPPD could be considered as one of the essential factors which could control the activity of the whole biosynthetic pathway due to its localization at the beginning of the biosynthetic pathway. HGGT is the only enzyme which differs in the biosynthetic pathway of tocopherols and tocotrienols and that is why it could cause changes in the composition of vitamin E. Seven barley cultivars with different vitamin E level were grown under controlled conditions and activity of HPPD and HGGT in ears was measured four, eight and twelve days after pollination. The activity of the HPPD gene corresponded with the final vitamin E content detected in grains (r = 0,75). There was a relationship between gene activity of HPPD eight and twelve days after pollination and vitamin E content, which was typical for these cultivars in field conditions (r = 0,85). The gene for HGGT had smaller correlation to tocotrienol content.
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