Monitoring of changes of ferulic acid content in brewing materials using the UPLC with PDA detector
2010
Belakova, S.,Vyzkumny Ustav Pivovarsky a Sladarsky, Brno (Czech Republic). Sladarsky Ustav | Benesova, K.,Vyzkumny Ustav Pivovarsky a Sladarsky, Brno (Czech Republic). Sladarsky Ustav | Mikulikova, R.,Vyzkumny Ustav Pivovarsky a Sladarsky, Brno (Czech Republic). Sladarsky Ustav | Svoboda, Z.,Vyzkumny Ustav Pivovarsky a Sladarsky, Brno (Czech Republic). Sladarsky Ustav
This study focused on monitoring the changes in ferulic acid concentration in the series barley - malt - wort. Twenty-one samples of barley varieties from four different localities were analyzed. Alcalic hydrolysis was used to release ferulic acid from the homogenized barley and malt samples. After adjusting pH, the extract was purified using the SPE. The optimized and validated Ultra Performance Liquid Chromatography with PDA detector was used for the analytical determination of ferulic acid in brewing materials. Ferulic acid concentration increased from 639.0-1,555.8 mg/kg in barley to 1,441.7-2,174.6 mg/kg in malt. In wort, it varied between 3.91 and 9.09 mg/L.
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