Study of genes involved in the intramuscular fat content and composition of muscles Longissimus dorsi and Gluteus medius in a Duroc population | Estudio de los genes implicados en la cantidad y composición de la grasa intramuscular de los músculos Longissimus dorsi y Gluteus medius en una población Duroc
2009
Solé, M., Institut de Recerca i Tecnologia Agroalimentàries, Lleida (España). Genètica i Millora Animal | Pena, R., Institut de Recerca i Tecnologia Agroalimentàries, Lleida (España). Genètica i Millora Animal | Amills, M., Universitat Autònoma de Barcelona, Bellaterra (España). Facultat de Veterinària | Cánovas, A., Institut de Recerca i Tecnologia Agroalimentàries, Lleida (España). Genètica i Millora Animal | Gallardo, D., Institut de Recerca i Tecnologia Agroalimentàries, Lleida (España). Genètica i Millora Animal | Reixach, J. | Díaz, I., Institut de Recerca i Tecnologia Agroalimentàries, Monells, Girona (España). Tecnologia dels Aliments | Noguera, J.L., Institut de Recerca i Tecnologia Agroalimentàries, Lleida (España). Genètica i Millora Animal | Quintanilla, R., Institut de Recerca i Tecnologia Agroalimentàries, Lleida (España). Genètica i Millora Animal
The intramuscular fat (IMF) content and composition are key traits in organoleptic, technological and nutritional quality of meat. We have conducted a study of genes influencing these traits in pigs by two approaches: QTL and expression analyses. The animal material consisted on 350 barrows of a commercial Duroc line, distributed in 5 half-sib families. The percentage of IMF, cholesterol content and fatty acid composition were determined in Longissimus dorsi and Gluteus medius muscles. A gene expression analysis of individuals with extreme values for several parameters related with lipid metabolism and fat meat characteristics was carried out in these two muscles by RNA hybridization in the GeneChip Porcine Genome Array (Affymetrix). All individuals were genotyped for 110 microsatellites distributed across the 18 autosomes. Several genome-wide significant QTL with effects on %IMF and several fatty acid profile have been detected at SSC 3, 6, 7, 12, 14 and 18. Moreover, we have detected 350 genes differentially expressed between the two analysed muscles, along with 579 genes differentially expressed in the two groups of animals with high and low levels of lipid metabolism. A list of potential candidate genes has been elaborated from genes differentially expressed and located in the confidence intervals of detected QTL.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria