Isolation and characterization of classical swine fever virus from pigs in Assam
2008
Sarma, D.K. | Mishra, N. | Rajkumar, K. | Sarma, S. | Singh, N.K.
Classical swine fever (CSF) virus in tissue samples of pigs slaughtei-ed for human consumption and from a diseased pig in Assam was isolated and characterized. The virus antigen was detected in tissue samples by sandwhich-ELISA. Seven of the 10 ELISA positive tissue samples reacted with "bovine viral diarrhoea virus monoclonal antibodies. CSF virus was isolated from 5 of the ELISA positive tissue samples in PK-15 cellline. In RT-PCR amplicons ofexpected :size 288bp could be generated from the ELISA positive sampIes. Sequence analysis of two samples one each from the slaughtered and diseased pigs confIrm the presence of CSF viral RNA
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