Influence of copper on embryo viability and development in hyacinth bean seeds
2010
Nautiyal, Nirmala | Awasthi, Sapna
An experiment was conducted to study the role of Cu in biosynthesis and accumulation of seed reserves and impact of exogenous ABA application on Cu deficiency. Fertilized ovules of hyacinth bean (Dolichos lablab L.), cultured aseptically at 8 and 10 days after anthesis developed copper deficiency after 5 days in culture at 0.02 ìM Cu resulting in reduction in seed reserve accumulation and loss in viability of embryos. The results suggest that decrease in the activities of acid invertase and Cu enzymes, ascorbate oxidase and polyphenol oxidase and induction of some Cu stress proteins resembling HSP70 affected the seed development in hyacinth bean at deficient Cu. Exogenous ABA application (0.1 mM) enhanced the level of some reserves but was unable to reverse the Cu deficiency effects.
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