Regeneration of sour cherry (Prunus ceraseus) through In-vitro propagation
2010
Dar, H.A. | Singh, S.R. | Srivastava, K.K. | Sharma, M.K. | Sundouri, A.S.
Dormant cutting procured from mature plants, were subjected to forcing treatment for in-vitro propagation. The culture media i.e., Murashige and Skoog medium (MS) with three strength levels viz., full, half and three fourth were used. Plant growth regulators viz. BAP (0.25 mg1−1, 0.50 mg1−1, 0.75 mg1−1) and IBA (0.00 mg1−1 and 0.01 mg1−1) were supplemented with MS media. The design of experiment was CRD with three replications. MS half strength basal medium supplemented with 0.50 mg l−1 BAP plus 0.01 mg l−1 IBA resulted in the highest survival percentage of in vitro cultures. MS medium supplemented with 1 mg l−1 BAP + 0.10 mg l−1 IBA fortified with 2 mg l−1 BAP + 0.10 mg l−1 IBA recorded the maximum proliferation efficiency. MS medium supplemented with BAP @ 0.1 mg l−1 and devoid of auxin recorded the optimum elongation of micro shoots. MS medium fortified with 2 mg l−1 IBA gave the highest rooting percentage and average number of roots per explant. The studies culminated in standardization of protocol for in vitro propagation of sour cherry rootstock.
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