In vitro selection of hill maize (Zea mays L.) hybrids for low phosphate tolerance
2010
Joshi, Rohit | Shukla, Alok | Kumar, Pramod
Genotypic response of hill maize hybrids viz. Vivek-4, Vivek-5, VL-42 and Him-129 to low concentrations of phosphate (low-P) was studied in vitro. An induction in anthocyanin accumulation and increase in total protein content were observed with decreasing concentration of P in the medium. SDSpolyacrylamide gel electrophoresis of in vitro cultured plants showed the over expression of 4_6 polypeptides under P deficient levels than to sufficient. Thus, results thereby indicated that many proteins to be specifically synthesized de novo under Pi deficiency as part of the adaptive mechanism under low P condition. Amongst the four hybrids of maize Him-129 and Vivek-5 showed relatively good response in terms of anthocyanin accumulation in stem and higher total protein content along with activation of few extra new bands of polypeptides under P deficiency and so, proved their tolerance against low P stress.
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