Improvement of culture media for Anthrax vaccine production.
2008
Mo`azzeni Jula, Gholam Reza | Jabbari, Ahmad Reza | Bani Hashemi, Reza
Anthrax is primarily a disease of herbivorous animals, although all mammals, including humans, and at least some avian species can contact it. Mortality can be high, especially in herbivores. The aetiological agent is the spore-forming, Gram-positive rod-shaped non-motile Bacillus anthracis. The disease has worldwide distribution and is a zoonosis. The most widely used vaccine for prevention of anthrax in animals was developed by Max Sterne in 1937, is a live, non-encapsulate, spore former held in suspension. The method of vaccine production briefly is: seed lots are cultured on soild medium in Roux flasks formulated to promote sporulation of the organisms, incubacted at 37oC for several days until cultures show at least 80% of the organisms to be in sporulated form when examined following staining for spores. The growth from each flask is then harvested with physiological saline. The high efficacy of vaccine in preventing the disease without any side effects in all animals leads to high demands for vaccine. This request for vaccine needs producing the vaccine during all the year. The aim of this study was to increase the yield of vaccine production by substitution of a culture media instead of N-Z case agar which is very expensive and make some difficulties in vaccine production. The results showed that substituting the tryptone instead of N-Z case agar and also decreasing the amount of tryptone up tp 20% of the original amount of N-Z case agar in culture medium of the vaccine production, leads to increasing the growth of organism and also increasing the sporulation of the organisms up to 100% and obviate the difficulties. Therefore, while the cost of vaccine production is decreased, its yield will be increased.
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