Investigation of change in morphological and physiological parameter induced by UV-A, UV-B and UV-C of ultraviolet radiation in colza seedling ( Brassica napus)
2003
Nasibi, F,, Shahid Bahonar, University of Kerman | Kalantari, Kh,, Shahid Bahonar, University of Kerman | Rashidi, M,, Shahid Bahonar, University of Kerman
Reduction in ozone layer and increase in ultraviolet radiation which reach the earth surface has been the subject of many researchers. In this study the effects of UV-A, UV-B and UV-C bands of ultraviolet on some growth parameter of colza`s seedling were investigated shoot length, root length, shoot and root dry weight were measured. Other parameter which were the subject of measurement in this experiments were, chlorophyll a, b and total chlorophyll,carotenoids, reducing sugar content of root and shoot and compound which absorb UV ( flavonoids and anthocyanins). In this investigation we found that shoot and root length, dry weight of shoot and root, which were irradiated either with UV-B or UV-C reduced significantly when compared with control. However leaf area in UV-B UV-C did not reduced significantly but dry weight did not decrease which possibly is indication of UV-absorbing compound which concentrated in leaf. Chlorophyll a, Chlorophyll b and total chlorophyll content in those plants which treated with UV (specially UV-B and UV-C ) reduce significantly in comparison with control. Reduction in reducing sugar specially in stem irradiated wi th UV could well be the indication of reduction in photosynthesis rate. In root we did not observe significant difference in reducing sugar. Measurement of compounds which absorb UV including flavonoids and anthoyanins showed that these compounds increased when plant irradiated with UV light specially in UV-B and UV-C treated plants. Key words: UV radiation, Anthocyanins, Flavonoids, Colza
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