Diversity of digestive enzymes and biochemical properties of major polysaccharide degrading enzyme of Haliotis discus discus
2009
Niwa, K., National Research Inst. of Fisheries Science, Yokosuka, Kanagawa (Japan) | Aono, H. | Sawabe, T.
In order to elucidate the nutritional physiology on Kuro abalone Haliotis discus discus, activities of digestive enzymes were measured, and the three major polysaccharide degrading enzymes were purified and characterized. Lipase, protease and polysaccharide degrading enzyme activities were detected from both digestive fluid and homogenate of digestive diverticulum of the abalone. The enzyme activities of alginate lyase, cellulase and mannanase were rather higher than the other enzyme activities. An alginate lyase, a cellulase and a mannanase were electrophoretically purified. MW of there purified enzymes were 27.5, 64.6, and 38.1 kDa, optimal temperatures were 40, 40 and 55degC, and optimal pH were 7.0, 5.0 and 5.0, respectively.
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