Production of the Acetylcholinesterase Inhibitor from Yarrowia lipolytica S-3
2008
Lee, D.H. (Paichai University, Daejeon, Republic of Korea) | Lee, J.S. (Paichai University, Daejeon, Republic of Korea) | Yi, S.H. (Korea Food Research Institute, Sungnam, Republic of Korea) | Lee, J.S. (Paichai University, Daejeon, Republic of Korea), E-mail: [email protected]
The acetylcholinesterase (AChE) inhibitor of Yarrowia lipolytica S-3 was maximally produced when it was incubated at 30℃ for 36 h in an optimal medium containing 1% yeast extract, 2% peptone and 2% glucose, with an initial pH 6.0. The final AChE inhibitory activity under these conditions was an IC∧50 value of 64mg/ml. After partial purification of the AChE inhibitor by means of systematic solvent extraction, the final IC∧50 value of the partially purified AChE inhibitor was 0.75 mg/ml. We prepared a test product by using the partially purified AChE inhibitor and then determined its stability for the development of a new antidementia commercial product. The test product was stable at room temperature for 15 weeks.
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