Antioxidation Effects of Rosmarinic Acid on Human Skin Melanoma Cells Treated with Hydrogen Peroxide
2009
Yoo, S.M., Dongkang College, Gwangju, Republic of Korea | Kang, J.R., Konkuk University, Seoul, Republic of Korea
The purpose of this study was to evaluate the antioxidant effect of rosmarinic acid on the cultured human skin melanoma cells injured by reactive oxygen species. The cytotoxicity and antioxidant effect were analyzed by XTT assay for cell adhesion activity, DPPH-radical scavenging activity, lactate dehydrogenase (LDH) activity after treated of cells with or without hydrogen peroxide (H₂O₂) for 1-10 h. H₂O₂ decreased the cell adhesion activity in cultured human melanoma cells in a dose- and time-dependent manner after cells were treated with 20-110 μM of H₂O₂ for 5-7 h. IC∧50 values of H₂O₂ by XTT assay were 90 μM, respectively. H₂O₂ increased LDH activity dose dependently. LDH∧50 was determined at 60 μM H₂O₂ after treatment of cells with 50-90 μM H₂O₂ for 8 h. Rosmarinic acid increases cell adhesion activity, DPPH-radical scavenging and decreases LDH activity. The treatment of the cells with H₂O₂ shows highly toxic effects on cultured human skin melanoma cells. Rosmarinic acid increases cell adhesion activity, DPPH-radical scavenging activity and decreases LDH activity in the cells treated with H₂O₂. It is considered that rosmarinic acid shows antioxidant effect on ROS such as H₂O₂.
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