Discrimination technology of fig (Ficus carica L.) cultivar 'Toyomitsuhime' using DNA markers
2010
Ikegami, H., Fukuoka-ken. Agricultural Research Center, Chikushino (Japan) | Nogata, H. | Awamura, M. | Hirashima, K.
We developed discrimination technology for 15 domestic and foreign fig (Ficus carica L.) cultivars including 'Toyomitsuhime' using DNA markers to protect intellectual property rights and to ensure an adequate post-market based on appropriate labeling of figs. We developed the SSR and STS methods. The former enables us to discriminate 'Toyomitsuhime' using only one SSR marker (LMFC20 or LMFC30) and even to identify all 15 cultivars reciprocally using four SSR markers (LMFC26, LMFC27, LMFC28, and LMFC30). By contrast, the latter provides two markers (TOP5, TOP2) that are applicable for multiplex PCR, which enables us to undertake simpler, faster, and less expensive discrimination of 'Toyomitsuhime' than the former in as little as 8 hours.
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