Effect of Kinetin on In Vitro Development of Parthenogenetic Porcine Oocytes Exposed to Demecolcine Prior to Activation
2009
Kim, K.Y., Seoul National University School of Dentistry, Seoul, Republic of Korea | Park, S.K., Seoul National University School of Dentistry, Seoul, Republic of Korea | Roh, S.H., Seoul National University School of Dentistry, Seoul, Republic of Korea
This study was designed to investigate the effect of kinetin on in vitro development of parthenogenetic porcine oocytes exposed to demecolcine prior to activation. In vitro matured metaphase Ⅱ stage oocytes were incubated in 0 or 2 ㎍/ml demecolcine supplemented defined culture medium for 3 h and the oocytes were activated electrically. The parthenogenetic porcine embryos were then cultured in 0 or 200 μM kinetin supplemented defined culture medium for 7 days. Regardless of demecolcine treatment, kinetin supplementation increased blastocyst rates significantly (7.0% versus 12.1% and 4.9% versus 8.5%; Control versus Kinetin and Demecolcine versus Kinetin + Demecolcine, respectively, p less than 0.05). Demecolcine treatment before activation tended to decrease blastocyst rates regardless of kinetin supplementation although it is not statistically significant. Total cell numbers in the blastocysts also tended to be elevated in embryos when supplemented with kinetin, however only the result between Kinetin and Demecolcine groups is statistically significant (37.6 ± 7.2 versus 28.1 ± 9.5, respectively, p less than 0.05). In conclusion, the present report shows that kinetin enhances developmental competence of parthenogenetic porcine embryo regardless of demecolcine pre-treatment before parthenogenetic activation when they were developed in defined culture condition.
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