Isolation and Identification of Entomopathogenic Fungus from the Pine Wilt Disease Vector, Monochamus alternatus Hope (Coleoptera: Cerambycidae) in Korea
2009
Shin, T.Y., Chungbuk National University, Cheongju, Republic of Korea | Choi, J.B., Chungbuk National University, Cheongju, Republic of Korea | Bae, S.M., Chungbuk National University, Cheongju, Republic of Korea | Cha, Y.R., Chungbuk National University, Cheongju, Republic of Korea | Oh, J.M., Chungbuk National University, Cheongju, Republic of Korea | Koo, H.N., Chungbuk National University, Cheongju, Republic of Korea | Woo, S.D., Chungbuk National University, Cheongju, Republic of Korea
Entomopathogenic fungi were isolated directly from a cadaver of adult Monochamus alternatus supporting fungal sporulation, using a semi-selective medium and then screened several fungal colonies. The pathogenicity of each fungus was tested using oak longicorn beetle, Moechotypa diphysis, as substitutive insect. As the result, only one of them showed high pathogenicity against M. diphysis, with up to 100% mortality within 21 days of inoculation. Selected fungus was named as MaW1 and identified by Beauveria bassiana using microscopic examination and DNA analysis. Pathogenicity was also evaluated to M. alternatus.
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