Antioxidant and Antimicrobial Activities of Various Solvent Fractions of Fine Ginseng Root
2009
Lim, J.K., Korea Polytechnic University, Siheung, Republic of Korea | Kang, H.J., Korea Polytechnic University, Siheung, Republic of Korea | Kang, S.N., Korea Polytechnic University, Siheung, Republic of Korea | Lee, B.Y., CHA University, Seongnam, Republic of Korea
This study was carried out to investigate the changes of yield, total phenolics, saponin content and composition, antimicrobial, and antioxidant activities of various fractions of fine ginseng root (Panax ginseng C.A. Mayer) by maceration method in the order of increasing polarity (hexane, chloroform, ethyl acetate, butanol, and water). Butanol fraction showed the highest total saponin content compare to other fractions. Hexane traction could harvest significantly high ginsenoside Rg2, Rg1, and Rf (p less than 0.05). And the contents of ginsenoside Rh1, R,,3, and Rg1 showed relatively higher in the fraction of ethyl acetate than other fractions. The system of hexane-chloroform-ethyl aceate-butanol showed relatively high content of ginsenoside Re, Rd, Rc, Rb3, and Rb1. However, the last fraction of water still remained lots of Rb2 content. The fraction of water was the highest phenolics. The 1,1-diphenyl-2-picryhydrazil, superoxide, and hydroxyl radical scavenging activity of water fraction was higher than the other fractions. In antimicrobial activity the fraction of hexane showed relatively high antimicrobial activity against Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus. Bacillus cereus, and Escherichia coli. And the fractions of the chloroform and ethyl acetate showed higher antimicrobial activities than the other samples in against P. aeruginosa and S. typhimurium.
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